User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/06/16

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From the article: "Bacterial Bioluminescence: Equilibrium Association Measurements, Quantum Yields, Reaction Kinetics, and Overall Reaction Scheme"

  • The FMNH2(reduced)-luciferase association constant is 3x10-4M-1 at room temperature an is significantly different from the kinetic reciprocal Michaelis-Menten constant.(1)
  • Bacterial bioluminescence involves three substrates: FMNH2, RCHO and O2. It produces: FMN, H2O2 and COOH.
  • A simple Michaelis-Menten model does not describe the transient kinetics of the oxidation of FMNH2 on the luciferase.