User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/06/15

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


From the article: "Kinetics of Light Emission and Oxygen Consumption by Bioluminescent Bacteria"

  • Oxygen consumption by luciferase has been evaluated to be 17% of the total respiration (1)
  • Luciferase, regulatory enzyme presenting a positive cooperative effect with oxygen. Michaelis constant for luciferase has been evaluated to be in the range of 20 to 65 nM O2.(1)
  • O2 concentrations needed to produce half-maximal light emission range between 0.015 and 0.700 microM(4)
  • Vibrio fisheri exhibits an intermediate pattern between a tonic and a phasic bacteria.(5)
  • The relationship between intensity of light emission and O2 is best observed in three phases: i) initial light O2 uptake rate ocurring at the burst of luminiscence. ii) steady-state conditions for both muninescence and O2 consumption [duration species and substrate-dependent]. iii)Sharp decrease in both parameters.(5)
  • Proportion of luciferase pathway to the total oxygen consumption has been evaluated to be 11.7+-2.1%(6)
  • FMNH2 has appeared to be mostly free when it is involved in the muninescent system. Luciferase has roved to have a stricly cytoplasmic localization[V.harveyi](8)
  • Cyanide at millimolar concentrations could form with free aldehyde an inhibitory complex of luciferase.