User:Daniel Goodman/Notebook/Weiss/2010/01/19

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Restriction and Gel of PhyB917 & PIF3

Samples First 3 PhyB917 Samples (37 deg C, 10 min) PIF 3 Sample (65 deg C, 10 min)

Made 75 ml agar gel:

  • 75ml TAE
  • .75 g Agarose
  • 7.5 ul SYBR-SAFE

Put SYBR after cooling, cooled in water bath, set gel ~20 min.

Note: forgot to swirl after adding sybr, though color looked uniform

Cut top of gel off, saved bottom in 4°C fridge in ziploc w/ name&date

Loading Gel:

Lane Sample Amt Loading
1 PIF3 Old* ~15ul 1.5ul
2 PIF3 ~15ul 1.5ul
3 Hyperladder 1 5 ul n/a
4-10 PhyB917 Samples 1-7* ~15ul 1.5ul

* One PIF3 and PhyB917 Samples 1-3 are new, the other PIF3 and 4-7 are recovered from freezer from Dec, may fail or be labelled incorrectly

Expected for PhyB917:

Cut with BstBI - two bands, 3579 and 5314

Expected for PIF3:

Cut with AvrII - two bands, 2164 and 4791

Gel Image

DBG-Chemidoc 2010-01-19 15hr 04min.jpg http://www.genoprice.com/hyperladder.png