User:Daniel Catt/Notebook/Protein Biofilms Exploration/2011/06/07
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Method: Copper(II) Nanoparticles?
Although the goal is to use chloroauric acid to form BSA-conjugated gold nanoparticles, a similar product could be formed using copper(II) ions instead. The reason for the temporary substitution would be economical, because the copper in the form of cupric acetate monohydrate is relatively cheap.
(The problem with the 'standard' non-cupric sample is that an extra 10mL of water was not added.)
-3g of zein -27mL of 95% ethanol mixed -900μL of glycerol (30% on zein weight)
†The copper solution was prepared experimentally with 1.9965g of Cu(CH3COO)2·H2O into 10mL of water. This would have made a 1M solution if the copper had dissolved. More water was added incrementally to bring the total volume to 60mL, which corresponds to a [Cu(CH3COO)2·H2O] of 0.16666..repeating mM, which is truncated here to the embarrassingly unwieldy 0.17mM.