User:Daniel B Rosen/Notebook/Methylation Sensor/2011/10/15

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Daniel B Rosen 19:35, 15 October 2011 (EDT)

Methylate dr002, dr004, dr006, dr008

  1. From yesterday
  2. 5 hour digest in PCR machine
  • Next time: nanodrop to determine concentration.

Maxiprep dr001, dr003, dr005, dr007

  1. Completed
  • Next time: nanodrop

Cloning dr009-12

mCherry into 2, 4, 6, 8 backbones

  1. 5 hour digest in pcr machine from yesterday
  2. SAP (shrimp anarctic phosphatase) on backbone
    1. 15 min 37C
    2. 1 uL enzyme
    3. 1/10 SAP buffer
    4. 5 min inactivation at 65C
  3. gel purification
  4. overnight ligation in PCR