User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/04/01

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Preparing Broth and Agar

Objective

During this lab session, solutions of LB Broth were prepared. A solution of Ampicilin was prepared to be added to the broth.

Procedure

The correct concentration for dissolving LB Broth Media into water is 25g per liter of water.

  1. 8 solutions were prepared
    1. 4 1L solutions were obtained by dissolving 25g of LB Broth Media in 1L water
    2. 4 25mL solutions were obtained by dissolving 625mg of LB Broth Media in 25mL water
  2. The solution are placed in the autoclave at 121 degrees C for an hour.