User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/01

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Goals

  1. Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
  2. Spin down gamma method HRP pellet
  3. Remake solutions so they are more concentrated

Procedures

  1. Making concentrated AuNP solutions
    1. Spin down AuNP solutions at 18,000 rpm for 3 hours
    2. Combine pellets and suspend in water
    3. Use new solution to make conjugates
  • Note color change for red to gray/black


Notes: Kinetics

  • Reduction of heme group
  • OPD, look for diamino...change of concentration over time, rates
  • Change in absorbance = change in concentration
  • Find molar absorptivity
  • Deoxygen/oxygen bound
  • Take off clear super, spin down until it doesn't show GNP activity