User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/03/18

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Gamma Method Myoglobin Coated AuNP

  1. A 1/10 solution of the reactant free nano particles was prepared from the stock solution
  2. 27mL of the AuNP solution was transferred to a beaker
  3. 1mL of 5mg/mL solution Myoglobin in H2O was added to the beaker
  4. The mixture was stirred at room temperature for 30 minutes
  5. The solution was then centrifuged for 30 minutes at 10,000rpm
  6. The supernatant and pellet were separated
  7. the pellet was resuspended in PBS solution with 0.1% BSA


Alpha HRP Pellet
Alpha HRP Pellet
This graph shows absorbance increases in the HRP Pellet over time

Alpha HRP supernatant
This graph shows absorbance increasing slightly in the HRP supernatant over time

Control. OPD and H202 only

This graph reveals what happens to absorbance over time when no HRP nanoparticles are present
Here there is only hydrogen peroxide and OPD