User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2013/10/08
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To observe and measure ADA turnover kinetics in the absence of an inhibitor. This work will be the basis of our comparison to ADA-AuNP turnover studies.
The spectra revealed a shift in the position of the peak. THe peak moved from 260nm to 250nm which is an indication of the formation of Inosine from Adenosine. Here is a graph that illustrates this shift over time.
From our past data, we know that the molar absorptivity at 260nm for Adenosine and Inosine are 14025 and 5254 respectively and the molar absorptivity of inosine at 250nm is 11007 From today's observed that, the molar absorptivity of adenosine at 250nm was 11884.2. Using these values we were able to calculate the concentrations of adenosine and inosine over time using a system of equations. The change in concentration from the initial to final concentrations was put on a scatter plot over time. Here is the resulting graph: