User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/27

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September 27, 2016 : UV-Vis III

Objective

  1. Run UV-Vis on gold nanoparticle solutions prepped 09.21.16

Description

UV-Vis

  1. Solutions prepped on 09.21.16 were collected for use on UV-Vis
  2. Each solution, individually, was added to the cuvette
  3. The cuvette was added to the UV-Vis machine and run from 800nm to 200nm
  4. Once the run was complete the cuvette was cleaned with 2 soap, 2 water, 2 HCl and 2 water flushes. Followed by a thorough drying.

Data

0.75 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Lysozymestock μL of Water
4 500 0 0 335 92 278 3795
5 50 0 0 335 92 278 4245
6 5 0 0 335 92 278 4290
7 0 0 0 335 92 278 4295
8 0 5 0 335 92 278 4290
9 0 50 0 335 92 278 4245
10 0 500 0 335 92 278 3795
11 0 0 5 335 92 278 4290
12 0 0 50 335 92 278 4245


1.25 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Lysozymestock μL of Water
4 500 0 0 335 153 278 3734
5 50 0 0 335 153 278 4184
6 5 0 0 335 153 278 4229
7 0 0 0 335 153 278 4234
8 0 5 0 335 153 278 4229
9 0 50 0 335 153 278 4184
10 0 500 0 335 153 278 3734
11 0 0 5 335 153 278 4229
12 0 0 50 335 153 278 4184
09272016 0.75Fructose WvA.png

09272016 0.75Fructose Absorbance.png

09272016 1.25mMFructoseWvA.png

09272016 1.25mMFructose Absorbance.png

Notes

Samples at pH 11 contained precipitate