User:Corey Bear/Notebook/(03 July 2014) Hay Infusion Culture

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Intent: This process is a follow on step from the original transect experiment underway. The intent of this process is to analyze the original transect derived from a predefined ecosystem located on American University. 100 microliters of content from the Hay Infusion Culture previously constructed were added to the first of four test tubes, then sequentially transferring 100 microliters to each test tube, from the one previous (this creates a dilution factor of: 10-2, 10-4, 10-6, 10-8), and then added to agars for a plating serial dilution.

Overview: 100 microliters of content from the Hay Infusion Culture previously constructed were added to the first of four test tubes, filled with 10 mililiters of sterile broth, then sequentially transferring 100 microliters to each test tube. Each tube had100 microliters added from the previous (exception of the first), which has a dilution factor of: 10E-2, 10E-4, 10E-6, and 10E-8. Two agar plats were prepared for each test tube, one which was sterile and one with tertracycline. Each plate had 100 micro liters added, and then stirred with a sterile glass rode. This created a dilution factor of: 10E-3, 10E-5, 10E-7, and 10E-9 Upon completion, the plates were placed near the window for further processing.

Observations: Samples of the Hay Infusion Culture were examined under a 40x compound microscope to identify potential organisms present prior to further analysis. The links below are a few organisms that were seen during this observation.



Paramecium Bursaria:

Peranema Sp:


Serial Dilution Diagram:

Additional observations: at first glance, the Hay Infusion Culture separated by density. This was seen through the segregation between the moldy top and thinker sediment bottom, with murky water in-between; an odor of decaying wetlands and moldy milk was also present. Moreover, organisms seen in the above links were motile and very present by the thousands in a single screening through a microscope. (sizes and descriptions of these organisms can be seen in the links provided.) Subsequently, these extant organisms fall within both the prokaryotic and Eukaryotic domains.

‘’’Predictions:’’’ If observations continued for additional months, the continued grow would permit under optimal conditions; pending nutrients and environmental factors remaining constant. This may yield an increase in the current colonies present in the Hay Infusion Culture.


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