new CK2
- Induction will be done at 2pm
- Take 2 500ul total induced samples, spin, wash, spin, resuspend
- Spin 10min 6K 4C in large Oakridge bottle, discard LB
- Wash in 25ml MK buffer, transfer to small Oakridge tube
- Spin 10min 6K 4C, discard MK buffer
- Resuspend in 30ml MK buffer + 300ul 100x protease inhibitor
- Store 5ml in a conical in -80C
- Store 25ml in a conical in -80C
Substrates
- Bad news: did PCR cleanups wrong, yielded very low concentrations
- Good news: calculations prove that there is enough PCR product to do T4 treatment for cloning
- Bad news: the pET-30 kit has not come in yet
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