User:Christine Doan/Notebook/Identifying Kinase Substrates of PfCK2/2014/02/18

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Primer Design

  • DC approved

Old CK2 DNA stock

  • Andrew made a regular and a digest DNA gel
  • Select two enzymes to digest miniprepped old CK2 DNA from stock
  • Verify size of digests before making dilutions to send off for sequencing
  • 20ul reaction
    • 1ul NdeI
    • 1ul XhoI
    • 2ul Buffer 4
    • 2ul 10x BSA
    • 5ul DNA (concentration >100ng/ul)
    • 9ul dH2O
  • Expected sizes: 1100 and 5370

Mystery

  • Adam's notebook reads:
    • pet29b, BL21 (DE3)
    • A box 7, #34
    • B box 7, #38
  • Glycerol stock reads:
    • A 5.31.01
    • B XLI Blue 5.28.01
  • DNA stock reads:

395 | CKII pDNA 105ng/ul 1/26/01

Mystery solved: Valerie Bracchi-Ricard cloned it into pET 29b