User:Charlynn Burgess/Notebook/ATP Synthesis/2011/11/28

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'ATP Synthesis (Making n(Bu4NH+)ATP from (Na+)ATP)'' Morning

Experimental Methods #1

  1. Made 1 L of 50mM (13.896g) of n(Bu4N)Cl in water
  2. Washed column with 250 mL of the n(Bu4N)Cl solution
  3. Dissolved .5g of (Na+)ATP in a very small amount of n(Bu4N)Cl. Just enough to dissolve the (Na+)ATP
  4. Run dissolved ATP solution through column until there is a small amount of solution right above the column
  5. Run ~ 200mL n(Bu4N)Cl solution from step 1 on column and collect 150mL
  6. Let solution from step 5 run under air for ~ 2days
  7. Run ~ 200mL of n(Bu4N)Cl from step 1 through column to wash and discard

Experimental Methods #2:

  1. Dissolve 2.23g (5mmol) ATP(Na+) in 50mL distilled water
  2. Washed column with 100 mL of H20
  3. Ensured water was neutral using pH paperAdded Na+(ATP) to top of column
  4. Added Na+(ATP) to top of column
  5. Allowed effluent to drip into stirred solution of 10mmol n(Bu4N)Cl in 20mL ETOH in ice
  6. Added 10 mL of distilled water to top of column while allowing effluent to run in n(Bu4N)Cl + ETOH solution
  7. Repeated once to ensure acid solution eluted
  8. Ran under air for 2 days

Observations # 1: - Dark yellow highly viscous compound with white particles throughout


Observations # 2: - Clear colored, extremely viscous, sticky compound


Observations #1 & 2 -As you can see, the clear compound is more viscous than the yellow compound.


Filtration of ATP-Ru Solution (Afternoon)


  1. Put together Vacuum Filtration
  2. Added RuATP solution to funnel
  3. Washed round bottom flask with DMF to obtain excess RuATP
  4. Added excess RuATP and DMF to filtration