User:Charlotte E. Vogler/Notebook/Lab 1: Biological Life at AU, Transect 2 on 7/1/14

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Lab 1: Biological Life at AU on July 1, 2014

Introduction, Purpose, and Objectives

Today we observed samples of organisms from the Volvocine Line. This included Chlamydomonas, Gonium, and Volvox. These organisms were observed to prepare us for the types of organisms we will find in our transect. We observed transect 2, an environment located on the campus of Wesley Theological Seminary. By studying a transect, our objective is to be able to make observations about the characteristics of our environmental niche so that we can later determine what types of organisms inhabit the area.

Materials and Methods

An 11.6 g sample was taken at the site of the transect. The sample included soil, pine needles, a small sample from ferns and ivies growing in the area on the ground and on the trunk of a tree. Back in the lab, a hay infusion culture was made with the 11.6g g soil sample, 0.1 g of dried milk, and 500 mL of filtered Deer Park water. The sample was mixed and left on a lab bench in an open jar to further mix and separate until samples could be taken from the culture at a later date.

Data and Observations

Transect 2 is a wooded area outside the campus of American University. It is approximately 20x20 feet in size. Below is a list of both abiotic and biotic characteristics of the area.

Biotic (living): 3 coniferous trees and 3 deciduous trees. Ground cover includes grass, pine needles, ferns, ivies, and weeds. Animals include gnats and mosquitos.

Abiotic (not living): there are no abiotic characteristics in this transect.

Photos of the transect can be seen below.

Transect Photos

Photo2Charlotte.jpg Photo3Charlotte.jpg Photo4Charlotte.jpg

From left to right: transect 2, pine needles cover the floor of the transect, and two large trees provide a shady cover to the area. Photo1Charlotte.jpg

A view of three of the six trees in the area and the ground plants that make up the transect. Photo5Charlotte.jpg

An aerial view of the transect provided by Google Maps.

Conclusions and Future Directions

In the second lab, we will observe samples from the mixed hay infusion culture to discover what organisms might be living in the ecosystem that makes up Transect 2.