User:Catherine Anania/Notebook/DNA Sequence

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The purpose of this lab was to identify a species of bacteria present in the farm transect, after extracting it from the Hay Infusion Culture, growing it on an agar plate and performing PCR to determine a DNA sequence. Unfortunately, this process didn't work, so instead we observed a sequence from bacteria collected in the spring. Below is a picture of our PCR results

Pcrresult.jpeg


A DNA sequence was given to us and used in the BLAST program run by NCBI. The sequence was found to belong to Chryseobacterium sp. OVC14 16S ribosomal RNA gene. Chryseobacterium is a gram negative genus of bacteria, named for its golden color. The genus includes many types, which are found everywhere from raw milk, to the Russian space station. It is very possible that we would have found a sample of chryseobacterium in our farm transect as well.


Sources:

jsm.asm.org/content/42/1/445.full.pdf

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