User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/10/19

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UV-Vis Procedure Solutions were prepared on 10/18/2016 The solutions had been heated in an 80C oven for four hours and then sat at room temperature. UV-Vis spectra were taken of each sample from 200-800nm. The test tubes were kept completely wrapped in tin foil the entire time, because AgNO3 is light sensitive. Observations As with gold, it is at pH 11 and pH 12 that unique spectra are seen. The solutions are more clear at pH 11 and pH 12 than for the other solutions. As fructose concentration increases, so does the cloudiness of the solutions for pH 11 and pH 12 pH 12 was also more clear for all fructose concentrations. Solutions All of the solutions for both concentrations of fructose were cloudy. Below are photos of pH 11 and 12 respectively, going in order from 0mM Fructose- 0.25mM fructose Ag NP's, myoglobin, pH11: 0mM, .0625mM, .125mM, .25mM Fructose Ag NP's, myoglobin, pH12: 0mM, .0625mM, .125mM, .25mM Fructose Data Graphs for .125mM Fructose Absorbance v. Wavelength Absorbance at 272nm Absorbance at 400nm Graphs for 0.25mM Fructose Absorbance v. Wavelength Absorbance at 396nm Absorbance at 284nm Future Experiments To Do When making UV-Vis next time, also remake 0mM fructose and 0.0625mM fructose pH 9 and pH 5 samples. Control experiments: myoglobin, gold, fructose, silver User:Matt Hartings/Notebook/AU Biomaterials Design Lab