Ocean Optics 9/21/16
- The pH 5 sample from 9/13/2016 was redone with the correct concentration of myoglobin.
Procedure
- Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 0.1mM HCl 3mL total
- Au 3.73mM
- (0.25mM)(3.0mL)=(XmL)(3.73mM)
- XmL= .201mL= 201uL Au
- Myoglobin 160uM
- (12.5uM)(3.0mL)=(XmL)(160uM)
- X=0.234mL= 234uL Myoglobin
- HCl 0.1mM
- (3.0mL)(0.01mM)=(1.0mM)(XmL)
- XmL=0.03mL= 30uL HCl
- Water
- Volume of water= 3000uL- 201uL-234uL-30uL= 2535uL
- Scans were taken at 80oC every 2 minutes for 3 hours
Results
- Scans
Absorbance Scan at pH5
Wavelength Scan at pH5
User:Matt Hartings/Notebook/AU Biomaterials Design Lab
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