User:Cassandra M Barrett/Notebook/Open Chromatin/2015/11/24

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pJET Sequencing

Purpose: to verify the inserts from the pJET LCR reactions

Methods:

Reconstitute pJET1.2 primers to 100uM. Dilute to 10uM working stocks

Set up for each sequencing reaction 6uL total, 1uL of primer, 150-200ng DNA, to 6uL with water

For SOLS submission:

https://sols.asu.edu/resources/core-laboratories

Fill in form 1, bring to Life Sciences Tower E, room s17