User:Cassandra M Barrett/Notebook/Open Chromatin/2015/11/13

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

LCR pJET Miniprep and Verification

Purpose: to verify if Gal4DB_mCh and ATF2 were successfully assembled in the pJET vector


Use the plasmid Miniprep kit to isolate plasmid DNA from all five colonies grown in liquid broth. Streak spots onto LB+AMP plates from each culture before miniprep. No primers in lab for pJET vector to verify insert. Will do PCR when they arrive along with possible sequencing.