User:Cassandra M Barrett/Notebook/Open Chromatin/2015/10/05

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LCR Plate Results

Colony Counts:

H20 (- control): no colonies MV10 (+ control): lawn LCR1: 1 mucoid colony LCR2: 2 fuzzy colonies LCR3: 2 mucoid colonies, 3 fuzzy colonies

Daniel says that mucoid morphology is typical of DH5alpha containing inherent amp resistance. Fuzzier colony type most likely marks gradient of resistance.

All 8 colonies from LCR plates were used to inoculate 4mL of LB+Amp for miniprepping. Four tubes were similarly inoculated from MV10 + control in order to obtain more plasmid for further experiments.