User:Cassandra M Barrett/Notebook/Open Chromatin/2015/09/12

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Gel Verification of LCR Parts

Purpose: to verify lengths of nucleotide sequences for LCR


Oligo bridges (diluted for LCR) and stocks for PCR of LCR parts run on 1% agrose gel for 35min at 110V to verify identity before amplification and reattempting LCR. (2uL sample and 4uL dye)



The image shows proper length of starting plasmids and small vague bands as expected for the oligo bridges. We will proceed with part amplification via PCR next time.

B1: LCRb_ATF2 to MV10 B2: LCRb_MV10 to Gal4DB_mCh B3: LCRb_mCh to ATF2