User:Carly M. Montanero/Notebook/CHEM-571/2013/10/01

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Biomaterials Design Lab: Fall 2013 Main project page
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Objective

To monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection.

From Dr. Hartings


Procedure

1. UV-Vis Absorbance of Reactants, Catalysts, and Products

  1. Take UV-Vis of horseradish peroxidase stock solution.
  2. Take UV-Vis of luminol of stock solution. The absorbance too high, so the stock solution was diluted 200x.
  3. Take UV-Vis of 3-aminophthalic acid.
  • Data not taken.

Figures



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