User:Carly M. Montanero/Notebook/CHEM-571/2013/09/18

Biomaterials Design Lab: Fall 2013

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Objective To perform a redox titration on HRP in order to determine the standard potential of this protein. Procedure The procedure was taken from Dr. Hartings notebook. Figures The class data was collected and analyzed: Data The percent oxidized was determined by using the absorbance peaks at 405.32 nm: [(Absorbance Value for data point- Absorbance Min)/(Absorbance Max- Absorbance Min)]*100 where the maximum absorbance was assumed to represent 100% oxidized and the minimum absorbance was assumed to represent 100% reduced. The percent oxidized was subtracted from 100 to calculate the percent reduced.