User:Carly M. Montanero/Notebook/CHEM-571/2013/09/18

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Objective

To perform a redox titration on HRP in order to determine the standard potential of this protein.

Procedure

The procedure was taken from Dr. Hartings notebook.

Figures

  • The class data was collected and analyzed:

CHEM571 09.18.13 potassiumHRP Echem REDOX table.png CHEM571 09.18.13 potassiumHRP Echem REDOX graph.png

Data

The percent oxidized was determined by using the absorbance peaks at 405.32 nm:

[(Absorbance Value for data point- Absorbance Min)/(Absorbance Max- Absorbance Min)]*100

  • where the maximum absorbance was assumed to represent 100% oxidized and the minimum absorbance was assumed to represent 100% reduced. The percent oxidized was subtracted from 100 to calculate the percent reduced.