User:Burak Yilmaz/Notebook/YR10 protein interaction/2009/09/22
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300 μl DNA Binding Buffer to 150 μl DNA sample). Transfer sample mixtures to the wells of a Zymo-Spin I-96 Plate™ mounted on a Collection Plate. Alternatively, add two volumes of DNA Binding Buffer to each volume of DNA sample directly in the wells of the Zymo-Spin I-96 Plate™ mounted on a Collection Plate. Ensure proper mixing by pipetting up and down a few times.
mixtures have been completely filtered. Discard the flow-through.
Centrifuge at 2,500-8,000 rpm for 5 minutes. Repeat wash step. (Alternatively, one wash can be performed using 600 μl Wash Buffer).
Zymo-Spin I-96 Plate™ onto an Elution Plate and centrifuge at 2,500-8,000 rpm for 5 minutes to elute the DNA. Ultra-pure DNA in water is now ready for use.