User:Burak Yilmaz/Notebook/YR10 protein interaction/2009/09/03

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Gel preperation running plasmid on gel,LB preperation for agro culture

  • incubate 20 ml agro cells in 250 ml flask

gel preperation

  • %1 agar
  • 50 ml TAE buffer
  • put in microwave

gel electrophoresis

  • 1.5 ul sample
  • 1.5 ul &x loading dye
  • 7.0 ul ddH2o
  • 80 volt 30-35 dk

The difference between microRNA valid target and the same microRNA computational non-valid target