User:Brian P. Josey/Notebook/2009/11/25
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Digestion of pBR322
We are going through the process of preparing the pBR322 from yesterday. Yesterday, we were able to digest it with EarI. Today, we are going to gel extract it at the proper EarI site and then ligate it into three pieces. To this affect, I created a gel with 0.4g of agrose and 50 mL of the TAE buffer.