User:Brady P. Dennison/Notebook/Notebook/2015/12/09

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Performing Restriction Enzyme Digest and Gel on GAL4DB/mch

Decided to use ‘notI’ Restriction Enzyme

Expected Cuts:

1192 Base Pairs
3243 Base Pairs

Digest (15 minutes -> 37 degrees):
DNA - 3 microliter
10X Buffer - 1.5 microliter
NotL - 1 microliter
Water - 9.5 microliter

Mch image.png