User:Bohao Liu/Notebook/Research/2012/09/19

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  • First run started 11:08: 13hr
    • Waited around 40s before starting run
    • Used column 2 on the plate with the 7th well being nonsorbitol bleaching curve
    • Takes 40 seconds to go through each round
    • I was retarded and used to 2M sorbitol in water
  • Second run started at 12:00 :14hr
    • Diluted cells 1 : 5
    • Waited 30 seconds after shock to start up again
    • Every 40 seconds for 80 mins
    • Used correct media
    • Klett is 14 for a 1:10 ratio


  • Third run started 2:00 : 16hr
    • Same as trial 2
    • Klett is 17 for 1:10 ratio
    • 2 cycles through before shock vs 1 normally
  • Fourth run started at 4:00 : 18 hr
    • Klett 24
    • Cells don't look as happy in this run