User:Benjamin Friedel/Notebook/CHEM 471/2016/04/13
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||
Objectives
ProcedureMaterials for Agar Disc Diffusion
-changed from Whatman membrane filter paper discs, these regular Whatman filter paper discs are MUCH more absorbent, with no leakage whatsoever, we probably should have been using these from the beginning -autoclaved in dry cycle
-used 4/12/16 reaction products for this set of disc diffusion tests
AMP Table
Disc Diffusion Procedure
-it was noted on 03/30/16 that loading 10ul onto the membrane filter paper discs did not result much less inhibition may be due to less volume loaded , this time load 15ul and see if there is still inhibition as to lessen the load on the filter paper discs from 20ul as well as increase load from 10ul
Note that this technique was conducted on the benchtop. Between each loading of a disc on a plate, the tweezers used were washed with EtOH to minimize contamination. However, the plates were open to the air for a period of time during plating and loading of discs. Because we are testing for bacterial inhibition by the nanoparticles and control, if there is contamination, but inhibition we may never know that there was contamination in the first place. Discussed with Dr. Hartings and protocol was approved. Checking the discs
|