User:Benjamin Friedel/Notebook/CHEM 471/2016/03/16

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Objectives

    1. UV Vis and Fluorescence Spectra for after 03/15/16 Rxn
    2. Data analysis for UV VIs and Fluorescence of 03/15/16 reaction

Materials

  1. Main UV Vis machine was used to record UVVIS Spectra of Casein AgNPs, not ocean optics.
    1. A water blank in the same quartz cuvette was record ed for blanking in data analysis

Procedure

  1. Note that UV Vis spectra of reaction mixes were taken from 200-800nm
  1. Fluorescence

-Fluorescence excited at 280nm for tryptophan and tyrosine fluorescence before and after reaction -scanned emission spectra from 300-540nm ( to avoid excitation peaks at 280 and 570nm)


  1. UV-Vis and Fluorescence Spectroscopy data can be found at the link SRB Lab Notebook Data

Results

  1. Color change notes and UV-VIs/ Fluorescence information can be found at the link SRB Lab Notebook Data
  1. Whey and Soy were in neutral reaction, Pea, Casein and Rice protein were basic - all experience color change (silver=yellow, red/purple = gold)