Objectives
- Work on Disc Diffusion Antibacterial Procedure and Prepare Reagents
- UV Vis on 02/24/16 Nanoparticle Reactions
Materials
- Ocean Optics UV Vis was used to record UVVIS Spectra of Casein AgNPs. The other machine broke and is unusable.
- Integration time= 10ms and Average =100.
- A water blank in the same quartz cuvette was subtracted from the spectra before running any samples.
Procedure
Materials for Agar Disc Diffusion
- Mueller Hinton Agar
-Dissolved Agar packet in 1L of water over hot plate
-Autoclaved on wet cycle
- Whatman Membrane Filter Paper
-autoclaved in dry cycle
- Protein-Nanoparticle samples and Ampicillin Control Dilutions
AMP Table
| Concentration (mg/L)
|
Concentration (ug/uL)
|
Volume (ul)
|
Mass (ug)
|
| 4 |
0.004 |
25 |
0.1
|
| 8 |
0.008 |
25 |
0.2
|
| 16 |
0.016 |
25 |
0.4
|
| 32 |
0.032 |
25 |
0.8
|
| 64 |
0.064 |
25 |
1.6
|
Example Nanoparticle Sample Dilutions
| Concentration (x)
|
| 1
|
| 0.5
|
| 0.25
|
| 0.125
|
| 0.0625
|
- Reaction mix data can be found at the link SRB Lab Notebook Data
Due to time constraints, the actual diffusion assay was not performed this day
| 
Project name
Main project page
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