User:Benjamin Friedel/Notebook/CHEM 471/2016/02/03

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Objective

Analyze results from 02/02/16 reactions (77 reactions), plan new reaction set for AgNP and AuNP formation with SPI, WPI, casein, pea protein, and brown rice protein. Record UVVis Spectra on successful NP reactions from 02/02/16.

Materials

  1. Used same nutritional proteins on 02/02/16
  2. Ocean Optics UV Vis was used to record UVVIS Spectra of Casein AgNPs. The other machine broke and is unusable.
    1. Integration time= 10ms and Average =100.
    2. A water blank in the same quartz cuvette was subtracted from the spectra before running any samples.
    3. Picture is shown on 02/02/16 page.

Procedure

Devised reaction set up to be prepared and ran on 02/02/16, not enough time today.

  1. Reducing stock protein concentrations (and thus concentrations in reaction mix) to increase metal/protein ratio to spur NP formation as we saw protein aggregate and nanofiber formation in previous reactions
  2. Hold protein volume and thus concentration) constant and change fructose concentration (reducing agent)

Detailed Reaction Setup for 02/02/16 Shown Below

1mL reactions ' ' ' ' ' ' ' '
2.5mM HAuCl4 (µL) 2.5mMAgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Au/Ag:Protein Notes
.5mg/ml Casein 1 40 0 560 0 400 1:15
2 40 0 440 0 520 1:11
3 40 0 360 0 600 1:09
4 40 0 280 0 680 1:07
5 40 0 200 0 760 1:05
6 0 40 560 0 400 1:15
7 0 40 360 0 600 1:09
8 0 40 200 0 760 1:07
1mg/ml SPI 9 40 0 560 0 400 1:15 Formed white fibrous bundles 9-11 (precipitate?)
10 40 0 440 0 520 1:11
11 40 0 360 0 600 1:09
12 40 0 280 0 680 1:07 purple nanofibers
13 40 0 200 0 760 1:05 purple nanofibers
14 0 40 560 0 600 1:15 14-22slighty cloudy, white accumulation at bottom (protein precipitate?)
15 0 40 560 20 580 1:15
16 0 40 560 40 560 1:15
17 0 40 360 0 600 1:09
18 0 40 360 20 580 1:09
19 0 40 360 40 560 1:09
20 0 40 200 0 600 1:07
21 0 40 200 20 580 1:07
22 0 40 200 40 560 1:07
1mg/ml WPI 23 40 0 560 0 400 1:15 *All whey samples have double volume of protein
24 40 0 440 0 520 1:11 23 (white precipitate at bottom)
25 40 0 360 0 600 1:09 (24-27) white stringy fibers climbing tube walls
26 40 0 280 0 680 1:07
27 40 0 200 0 760 1:05
28 0 40 560 0 600 1:15 28-30 clear
29 0 40 560 20 580 1:15
30 0 40 560 40 560 1:15
31 0 40 360 0 600 1:09 31-33 clear
32 0 40 360 20 580 1:09
33 0 40 360 40 560 1:09
34 0 40 200 0 600 1:07 34-36 clear
35 0 40 200 20 580 1:07
36 0 40 200 40 560 1:07
1mg/ml Pea Prot 37 40 0 560 0 400 1:15 37-40 purple and white fibers
38 40 0 440 0 520 1:11
39 40 0 360 0 600 1:09
40 40 0 280 0 680 1:07
41 40 0 200 0 760 1:05 41 only purple fibers
42 0 40 560 0 600 1:15 42-44 white precipitate at bottom
43 0 40 560 20 580 1:15
44 0 40 560 40 560 1:15
45 0 40 360 0 600 1:09 45-47 white precipitate at bottom
46 0 40 360 20 580 1:09
47 0 40 360 40 560 1:09
48 0 40 200 0 600 1:07 48-50 white preciptate at bottom
49 0 40 200 20 580 1:07
50 0 40 200 40 560 1:07
1mg/ml Brown Rice Prot 51 40 0 560 0 400 1:15 51-55 white precipitate with purple fibers near bottom of tube
52 40 0 440 0 520 1:11
53 40 0 360 0 600 1:09
54 40 0 280 0 680 1:07
55 40 0 200 0 760 1:05
56 0 40 560 0 600 1:15 56-58 white preciptate at bottom of tube and white particles (dots, fibers) clinging to walls
57 0 40 560 20 580 1:15
58 0 40 560 40 560 1:15
59 0 40 360 0 600 1:09 59 - 60 white preciptate at bottom of tube and white particles (dots, fibers) clinging to walls
60 0 40 360 20 580 1:09
61 0 40 360 40 560 1:09 61 tan precipitate at bottom of tube, no fibers clinging to the sides
62 0 40 200 0 600 1:07 59 - 60 white/yellow/black preciptate at bottom of tube and white particles (dots, fibers) clinging to walls
63 0 40 200 20 580 1:07
64 0 40 200 40 560 1:07