User:Benjamin Friedel/Notebook/CHEM 471/2016/02/02

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Objective

The objective for this experiment is to prepare gold and silver nanoparticles using nutritional soy protein isolate, micellar casein, whey protein isolate, pea protein isolate and brown rice protein isolate

Materials

Soy Protein Isolate (NutraBio) - http://www.nutrabio.com/category/spi/ Whey Protein Isolate (NutraBio) - http://www.nutrabio.com/product/WPI2/ Micellar Casein (NutraBio) - http://www.nutrabio.com/product/CAS2/ Pea Protein Isolate (Swanson Health Products) http://www.swansonvitamins.com/swanson-organic-certified-organic-pea-protein-powder-non-gmo-1-lb-454-grams-pwdr#label Sprouted Brown Rice Protein (Naked Nutrition) - http://nkdnutrition.com/products/organic-brown-rice-protein-powder

Protein concentrations are detailed on reaction setup. Concentrations were determined accurately through changing volume for a particular mass.

Procedure

This is the first day testing these nutritional proteins, the procedure from 01/27/16 is copied with these proteins as a starting point.

Reaction Setup Detailed below

' ' 2.5mM HAuCl4 (µL) 2.5mMAgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Au/Ag:Protein Notes
1 40 0 560 0 400 1:15
2 40 0 440 0 520 1:11
3 40 0 360 0 600 1:09
4 40 0 280 0 680 1:07
5 40 0 200 0 760 1:05
6 0 40 560 0 400 1:15
7 0 40 360 0 600 1:09
8 0 40 200 0 760 1:07
9 40 0 560 0 400 1:15 Formed white fibrous bundles 9-11 (precipitate?)
10 40 0 440 0 520 1:11
11 40 0 360 0 600 1:09
12 40 0 280 0 680 1:07 purple nanofibers
13 40 0 200 0 760 1:05 purple nanofibers
14 0 40 560 0 600 1:15 14-22slighty cloudy, white accumulation at bottom (protein precipitate?)
15 0 40 560 20 580 1:15
16 0 40 560 40 560 1:15
17 0 40 360 0 600 1:09
18 0 40 360 20 580 1:09
19 0 40 360 40 560 1:09
20 0 40 200 0 600 1:07
21 0 40 200 20 580 1:07
22 0 40 200 40 560 1:07
23 40 0 560 0 400 1:15 *All whey samples have double volume of protein
24 40 0 440 0 520 1:11 23 (white precipitate at bottom)
25 40 0 360 0 600 1:09 (24-27) white stringy fibers climbing tube walls
26 40 0 280 0 680 1:07
27 40 0 200 0 760 1:05
28 0 40 560 0 600 1:15 28-30 clear
29 0 40 560 20 580 1:15
30 0 40 560 40 560 1:15
31 0 40 360 0 600 1:09 31-33 clear
32 0 40 360 20 580 1:09
33 0 40 360 40 560 1:09
34 0 40 200 0 600 1:07 34-36 clear
35 0 40 200 20 580 1:07
36 0 40 200 40 560 1:07
37 40 0 560 0 400 1:15 37-40 purple and white fibers
38 40 0 440 0 520 1:11
39 40 0 360 0 600 1:09
40 40 0 280 0 680 1:07
41 40 0 200 0 760 1:05 41 only purple fibers
42 0 40 560 0 600 1:15 42-44 white precipitate at bottom
43 0 40 560 20 580 1:15
44 0 40 560 40 560 1:15
45 0 40 360 0 600 1:09 45-47 white precipitate at bottom
46 0 40 360 20 580 1:09
47 0 40 360 40 560 1:09
48 0 40 200 0 600 1:07 48-50 white precipitate at bottom
49 0 40 200 20 580 1:07
50 0 40 200 40 560 1:07
51 40 0 560 0 400 1:15 51-55 white precipitate with purple fibers near bottom of tube
52 40 0 440 0 520 1:11
53 40 0 360 0 600 1:09
54 40 0 280 0 680 1:07
55 40 0 200 0 760 1:05
56 0 40 560 0 600 1:15 56-58 white precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
57 0 40 560 20 580 1:15
58 0 40 560 40 560 1:15
59 0 40 360 0 600 1:09 59 - 60 white precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
60 0 40 360 20 580 1:09
61 0 40 360 40 560 1:09 61 tan precipitate at bottom of tube, no fibers clinging to the sides
62 0 40 200 0 600 1:07 59 - 60 white/yellow/black precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
63 0 40 200 20 580 1:07
64 0 40 200 40 560 1:07
Whey 2.5mM HAuCl4 (µL) 2.5mMAgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Notes
23A 40 0 560 0 400 23A white fibers
24A 40 0 440 0 520 24A white fibers
25A 40 0 360 0 600 25A purple nanoparticles with dark pellet at bottom
26A 40 0 280 0 680 26A light purple (white and purple) nanofibers at bottom
27A 40 0 200 0 760 27A slightly pink solution nanoparticles
28A 0 40 560 0 600 28-33 clear
29A 0 40 560 20 580
30A 0 40 560 40 560
31A 0 40 360 0 600
32A 0 40 360 20 580
33A 0 40 360 40 560
34A 0 40 200 0 600 34A, cloudy white fibers at bottom of tube
35A 0 40 200 20 580 clear
36A 0 40 200 40 560 clear

Results

Graphs for particular gold and silver NP species by protein are shown below

  1. One new color type indicative of AuNP was made, purple Whey AuNP Solution, however, when UV vis spectra was taken, noting appeared. Its possible that protein did not dissolve fully and thus only very few NPs were made


Figure 1: UVVIS Spectra for CaseinAgNP Synthesized 02/02/15
2.2.16 Casein AgNP.png

Note CaseinAg NP for this reaction time (4 hours 80˚C) peak at 420nm