User:Benjamin Friedel/Notebook/472 CHEM/2016/02/02

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Objective

Todays objective is to prepare gold and silver nanoparticles using pure casein and soy protein isolate as capping and reducing agents

Materials

Stock Solutions

  1. SPI (ARCON S 066400)
  1. Casein (amresco E666-500G)
    1. note store in refrigerator
  1. Fructose
  1. Choloroauric Acid (REM Chemicals)
    1. note store in desiccator, sensitive to metals
  1. Silver Nitrate (CMI 7985)
    1. note store in desiccator, sensitive to light

Procedure

The reaction spreadsheet is shown below. After creating the reaction mixes, samples were heated at 80˚C for 4 hours in the HERATHERM oven in Dr. Harting's Lab in Beeghly (on AU campus). Aluminum foil was used to cover the tubes. This reaction cycle also consisted of 30 minutes of heating and cooling beforehand. Samples were retrieved the next day (1/27/16), checked for color change and then covered in tin foil completely (to prevent oxidation by light of potentially unreacted silver nitrate).

1mL reactions ' ' ' ' ' ' ' ' ' Whey 2.5mM HAuCl4 (µL) 2.5mMAgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Notes
2.5mM HAuCl4 (µL) 2.5mMAgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Au/Ag:Protein Notes 23A 40 0 560 0 400 23A white fibers
1 40 0 560 0 400 1:15 24A 40 0 440 0 520 24A white fibers
2 40 0 440 0 520 1:11 25A 40 0 360 0 600 25A purple nanoparticles with dark pellet at bottom
3 40 0 360 0 600 1:09 26A 40 0 280 0 680 26A light purple (white and purple) nanofibers at bottom
4 40 0 280 0 680 1:07 27A 40 0 200 0 760 27A slightly pink solution nanoparticles
5 40 0 200 0 760 1:05 28A 0 40 560 0 600 28-33 clear
6 0 40 560 0 400 1:15 29A 0 40 560 20 580
7 0 40 360 0 600 1:09 30A 0 40 560 40 560
8 0 40 200 0 760 1:07 31A 0 40 360 0 600
9 40 0 560 0 400 1:15 Formed white fibrous bundles 9-11 (precipitate?) 32A 0 40 360 20 580
10 40 0 440 0 520 1:11 33A 0 40 360 40 560
11 40 0 360 0 600 1:09 34A 0 40 200 0 600 34A, cloudy white fibers at bottom of tube
12 40 0 280 0 680 1:07 purple nanofibers 35A 0 40 200 20 580 clear
13 40 0 200 0 760 1:05 purple nanofibers 36A 0 40 200 40 560 clear
14 0 40 560 0 600 1:15 14-22slighty cloudy, white accumulation at bottom (protein precipitate?)
15 0 40 560 20 580 1:15
16 0 40 560 40 560 1:15
17 0 40 360 0 600 1:09
18 0 40 360 20 580 1:09
19 0 40 360 40 560 1:09
20 0 40 200 0 600 1:07
21 0 40 200 20 580 1:07
22 0 40 200 40 560 1:07
23 40 0 560 0 400 1:15 *All whey samples have double volume of protein
24 40 0 440 0 520 1:11 23 (white precipitate at bottom)
25 40 0 360 0 600 1:09 (24-27) white stringy fibers climbing tube walls
26 40 0 280 0 680 1:07
27 40 0 200 0 760 1:05
28 0 40 560 0 600 1:15 28-30 clear
29 0 40 560 20 580 1:15
30 0 40 560 40 560 1:15
31 0 40 360 0 600 1:09 31-33 clear
32 0 40 360 20 580 1:09
33 0 40 360 40 560 1:09
34 0 40 200 0 600 1:07 34-36 clear
35 0 40 200 20 580 1:07
36 0 40 200 40 560 1:07
37 40 0 560 0 400 1:15 37-40 purple and white fibers
38 40 0 440 0 520 1:11
39 40 0 360 0 600 1:09
40 40 0 280 0 680 1:07
41 40 0 200 0 760 1:05 41 only purple fibers
42 0 40 560 0 600 1:15 42-44 white precipitate at bottom
43 0 40 560 20 580 1:15
44 0 40 560 40 560 1:15
45 0 40 360 0 600 1:09 45-47 white precipitate at bottom
46 0 40 360 20 580 1:09
47 0 40 360 40 560 1:09
48 0 40 200 0 600 1:07 48-50 white preciptate at bottom
49 0 40 200 20 580 1:07
50 0 40 200 40 560 1:07
51 40 0 560 0 400 1:15 51-55 white precipitate with purple fibers near bottom of tube
52 40 0 440 0 520 1:11