User:Benjamin D. Eachus/Notebook/Phosphorylation/2012/08/15

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  • Today's work was performed to replicate the creation of a gel using the centrifuge.
  • 15g (0.05397mol) of t-butylammonium chloride was dissolved in 20mL of H2O. Then, half of the solution was poured into a round-bottom flask, so each contained equal amounts of the mixture (~18mL). The contents of the round-bottom flask were rotovapped, until just 8mL of the solution remained. Then, 0.157g of ATP (0.02699mol) was added to the rotovapped solution. The ATP was stirred using a stir bar for 2 hours. The ATP did not dissolve.
  • The other beaker containing ~18mL of solution was kept over low heat on a hot plate to evaporate excess H2O. 0.157g of ATP was added to the mixture and dissolved. 4.4mL of EtOH was added to the solution and stirred. The mixture immediately turned white and cloudy, which lasted only 20 seconds. Then, 12 drops of 4M NaOH were added to the mixture. The mixture was continuously stirred. It was kept over heat until the mixture turned a clear, orange/yellow color. The solution was removed from heat at 2.699M t-butylammonium chloride. 4 mL of the solution was transferred to a test tube and placed into the centrifuge. The tube was stun at 3500rpm @ 4C for one hour. The tube was then removed. The solution had successfully gelled, but remained as a gel for just 30 seconds at room temperature. The remaining sample of orange gel was reheated until it reached a molarity of 3.37M. 4 mL of this solution was added to a test tube and spun at 3500rpm @ 4C for one hour. The mixture was a solid and remained a solid at room temperature.
  • The ideal molarity of t-butylammonium ammonium chloride for the gelation of the solution is ~3.00M.