User:Beatriz Gimenez De C./Notebook/572/2015/04/07

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  • Prepare solutions for SDS-PAGE analysis
    • 50 mM Glycine-HCl, pH 3: prepared by dissolving 0.380 g of glycine in 100 mL of water with the addition of 3 M HCl dropwise to achieve the desired pH
    • Running buffer
    • Prepare Sample
      • two samples were made for each time. One will be normal and the other will be diluted
        • 10 µL of each original reaction sample was diluted to 1 mL with Gly-HCl buffer
        • 10 µL of the original reaction sample in addition to 10 µL of the running buffer, skipping the dilution step
        • The last timepoint was used for each concentration (1 µM, 100 nM, 10 nM, 1 nM)
      • 10 µL of the glycine-diluted sample was further diluted with 10 µL of SDS-PAGE running buffer