User:Beatriz Gimenez De C./Notebook/572/2015/03/03

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Tasks

  • Bradford Analysis
  • Run Bradford analysis of the trypsin samples run Wednesday 25
    • 200μM of a 1 in 4 Bradford dilution (1 mL Bradford in 3 mL of Tris/NaCl buffer)
    • 150μM of each Chymotrypsin sample
    • 550μM of tris/NaCl buffer
  • Trypsin kinetics

Results

  • Kinetics of Chymotropsin at 1μM, 100nM, 10nM and 1nM

1uM.Chymotrypsin.Kinetics.png

100nM.Chymotrypsin.Kinetics.png

10nM.Chymotrypsin.Kinetics.png

1nM.Chymotrypsin.Kinetics.png