User:Beatriz Gimenez De C./Notebook/572/2015/02/25

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Tasks

  • Chymotrypsin 4 reactions will be monitored
    • 24 μM Stock solution 0.00060 g of Chymotrypsin in 1 mL of Tris/NaCl buffer
      • 0.208 mL of stock solution + 4.792 mL of Tris/NaCl buffer --> 1 μM
      • 0.020(8) mL of stock solution + 4.979 mL of Tris/NaCl buffer --> 100 nM
      • 0.002(1) mL of stock solution + 4.998 mL of Tris/NaCl buffer --> 10 nM
      • 0.020(8) mL of dilution (10 μL of stock solution in 990 μL of Tris/NaCl buffer) + 4.979 mL of Tris/NaCl buffer --> 1 nM
    • Fibers centrifuge (300 RPM for 10 min) + liquid was extracted.
    • Different protease concentrations added + samples were left in the incubator shaker (37C) for 2.5 hours.
    • Time points collected: 0, 30 min, 60 min, 90 min, 120 min, 150 min and 180 min
  • Preparation of more AuNP Fibers
    • Prepare Lysozyme 20 μM stock solution
      • Measured 0.01439 g of lysozyme into a 50 mL volumetric flask and filled the rest with distilled water
    • Prepare 2.5×10-3 M HAuCl4 stock solution
      • added 0.04361 g of HAuCl4 into a 50 mL volumetric flask and filled the rest with distilled water
    • Transfer 0.5 mL 2.5×10-3 M HAuCl4 into 29 culture tubes
      • 0.5×10-3(L)*2.5×10-3(mol/L)= 1.25 (mol) needed for a 45:1 ratio
    • Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes
      • 2.77777778×10-8(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio
    • Fill up 29 culture tubes to 5 mL total with distilled water
    • Placed in oven at 80ºC for 4h