User:Beatriz Gimenez De C./Notebook/572/2015/02/24

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Tasks

  • Run Bradford analysis of the trypsin samples run Wednesday 18
    • 200μM of a 1 in 4 Bradford dilution (1 mL Bradford in 3 mL of Tris/NaCl buffer)
    • 150μM of each trypsin sample
    • 550μM of tris/NaCl buffer

Results

  • Bradford Kinetics for 1μM, 100nM, 10nM and 1nM from 0min to 180 min.

1uMTrypsin.png 100nM Trypsin kinetics.png 10nM Trypsin kinetics.png 1nMTrypsin.png



Note

  • Gas Chromatography could not be used