Tasklist
- Analyze data of dialyzed 30:1 Colloid Solutions against CaCl2 set on October 21.
- Bradford Analysis
- 100 μL sample + 200 μL Bradford Reagent diluted (1:3) + 700 μL 50 mM Tris/ 50 mM NaCl buffer.
- Run UV-Vis between 400 nm and 800 nm
- UV-Vis analysis
- Fluorescence analysis.
- Ca2+ ISE
| KI concentration
|
mV measurement (mV)
|
| 2mM |
-198.3
|
| 5 mM |
-217.3
|
| 10 mM |
-237.0
|
| 25 mM |
-247.4
|
| 50mM |
-278.0
|
- Prepare dialysis of 0.6 g/L Lysozyme vs KI
- Use 3500 MWCO tubing
- Add 1 mL 0.6 g/L Lysozyme solution to 5 cells on one side of chamber
- Add 1 mL 2 mM, 5 mM, 10 mM, 25 mM, and 50 mM KI to the opposite side of the chamber (one concentration per cell)
- Follow protocols previously used
Note
- Used 0.6 g/ L Lysozyme prepared by Monika
|
Project name
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