User:Beatriz Gimenez De C./Notebook/571/2014/09/24

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  • continue analysis of dialysis data of 9/23/2014
    • Bradford analysis

9-23 dyalysis bard data.png

  • Prepare 0.5M acetic acid and 0.3M Na2SO4 solution (14.3 mL of acetic acid, 21.3060g were aded to 500 mL volumetric flask and the rest was filled with HPLC water)
  • Make dilutions of 1g/L of lysozyme to 0.5 g/L and 0.1 g/L lysozyme.
  • Run HPLC with a GPC (Gel Permeation Chromatography) column.
    • Ran MEMBRANE FILTRATION of solvent prior to running HPLC,
    • Filtered 0.5 M HAc 0.3 M NaSO4 under a vacuum to ensure all particles were <0.45 microns to degas (get rid of CO2 gas)
    • Set HPLC machine to: Waters 1525 Binary HPLC pump, flow of A at 0.8 mL/min
    • Used syringe filters prior to the injection for lysozyme and standard samples.