User:Beatriz Gimenez De C./Notebook/571/2014/09/17

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Task

  • Make dilution of HCL stock solution and Glycine stock solution (made last class)
  • Record conductivity and pH solutions
  • Make a pH and conductivity curve
  • Prepare and run SDS-PAGE Running-Buffer
  • Analyze dialyzed solutions from yesterday

Dilutions

  • Made 8 dilutions in 14 mL Falcon tubes using the volumes of stock solution and water. For each stock solution
  • Diluted ×2, 5, 10, 20, 50, 100, 200, 500.
Dilutions Nacl molarity (mM) pH conductivity (μS/cm)
2 25 6.064 3030
5 10 5.963 1178
10 5 6.189 623
20 2.5 5.989 304
50 1 6.188 290.2
100 0.5 6.507 68
200 0.25 6.331 75.4
500 0.1 6.768 50.8
Glycine dilution Molarity (mM) pH Conductivity (μS/cm)
2 25 3.479 383
5 10 3.6 201.1
10 5 3.704 130.5
20 2.5 3.835 93.9
50 1 4.282 43.8
100 0.5 4.336 35.6
200 0.25 4.762 25.98
500 0.1 5.381 86
  • Plots:

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