User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/07/31

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  1. The hybridization buffer turned somewhat blue overnight.
  2. I took the blot out of the hyb buffer and put it down on Saran wrap
  3. I added a ml of ECF and left them in the dark for about 5 min
  4. I imaged and could definitely see stuff but no evidence that I could see the dots. I rewrapped it in Saran wrap and left it back in the drawer
  5. Probably want to wash this when I can (once I've figured out what a non-stringent wash would look like).
  6. I used the primary wash buffer at room temperature. Following that I added more ECF.

Turns out that methylene blue can be used to stain nucleic acid membranes. I used a protocol I found on the Nature Methods site.