User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/07/28

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  1. Put ~400ng of DNA on fresh membrane.
  2. Crosslinked for 5min on big UV at high power.
  3. Bathed in EtBr.
  4. Could clearly see dots
  5. Does this work on old membrane?
  6. Not one that has been imaged already, there is some white precipitate and the gel doesn't turn red under the UV the way the others did.
  7. Tried a membrane that has been transferred but nothing else.
  8. Didn't look "white" like the imaged membrane, but couldn't see the dots.
    • Is this due to buffer, or what.
    • Do test with the 20x SSC.