User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/07/02

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  • Obtain detection substrate from Bryan
  • Order chemifluorescent detection substrate.
  • Research in vitro transcription to design better standards.

Primer design information

>wt AGGGGAACCTGCGGTTGGATCACCTCCTTA

>mutant AGGGGAACCTGCGGTTGGATCATTGTGGTA

wt16sProbe1

  • 5'-GGT TGG ATC ACC TCC-3' 49

wt16sProbe2

  • 5'-GTT GGA TCA CCT CCT TA-3' 48
  • reverse complement - TAAGGAGGTGATCCAAC

o16sProbe1

  • 5'-GTT GGA TCA TTG TGG-3' 44

o16sProbe2

  • 5'-GTT GGA TCA TTG TGG TTA-3' 47 - ordered this
  • reverse complement - TAACCACAATGATCCAAC
  • reverse complement - TACCACAATGATCCAAC - ordered this