User:Asya L. Tucker/Notebook/Asya 571/2015/10/27

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Objective

To run an experiment using 100 nM Trypsin and Au fibers on the Ocean Optics spectrometer.

Procedure

  • Ocean Optics
  1. Centrifuged falcon tubes containing gold fibers.
  2. Removed water with pipette.
  3. Prepare the sample that goes in the 3mL cuvette that will be used for ocean optics.
  4. The sample must contain:
    1. Gold fibers
    2. 2.996mL of Tris buffer
    3. 0.003994mL of trypsin, calculations are below
    4. The trypsin was added last, at the second reading such that second reading becomes time 0.
  5. We ran our sample overnight.
  6. Readings were taken every 30 seconds for 40 minutes, every 2 minutes until the sample had run for 2 hours and and then every 20 minutes overnight. The data was uploaded directly to DropBox for later analysis.

Calculations for volume of trypsin, and volume of buffer

    C1·V1 = C2·V2,
     where C1 = 75.11µM
           C2 = 0.1µM 
           V2 = 3mL
    V1 = 0.003994mL 
    Volume of buffer: 3mL-0.003994mL = 2.996mL

Data

RAM OceanOptics 100 nMTrypsin.png

Results