User:Ashlee Forbes/Notebook/0703 - T5 - Hay Culture & Protists

From OpenWetWare
Jump to: navigation, search

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Ashlee Forbes/Notebook/0703 - T5 - Hay Culture & Protists</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Owwnotebook icon.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages Help.png

Observing Protists in Transect 5

The purpose of the experiment detailed in this notebook was to observe protists in a hay culture. The hay culture was made from a soil and vegetation sample taken from Transect 5, a certified wildlife habitat on the campus of American University in Washington, DC. Because of the site’s unique status, the expectation here will be to see a large amount of protest life in the sample. A large amount of biotic life should be observed if the site is planted specifically to encourage wildlife. The hypothesis for this experiment will be: If samples of a hay culture from Transect 5 are observed, then a large number of protists will be observed.

Materials and Methods For this experiment, a 50 ml conical tube was used to take a soil and vegetation sample. Sample was collected from various areas around the transect, and comprised of ground mosses, grass, and topsoil. The sample was then mixed to combine components thoroughly.

In the lab, a Hay Infusion Culture was created using the soil sample. Eleven grams of the soil and vegetation sample were weighed and put into a large glass jar along with 500 ml of Deer Park water. To this, 0.1 grams of milk powder were added to the soil solution. The jar was then sealed, shaken to mix, then left uncovered for two days. After two days, a pipette dropper was used to pull samples from the culture. Samples were taken from the very top layer and from the bottom of the infusion, just about the solid layer. Specimens were observed at both 10x and 40x magnification using compound microscopes.

Data and Observations After two days, the hay culture was showing clear signs of decomposition. The jar had a pungent rotting smell and pieces of vegetation were blackened and floating on the surface. A light scummy film had begun to coat the water surface as well (Figure 1).

Ashlee Forbes - Hay Infusion.jpg Figure 1: Hay Infusion after 2 days

Under microscopic observation, however, there was a noted absence of life. The sample drawn from the top layer of the infusion did not contain any protists, although some plant cells were observed, though none were algae-based. The sample taken from the bottom layer returned slightly better results, with a few motile paramecium in evidence (Figure 2). These measured approximately 75 µm in length. Paramecium were identified with the aid of a dichotomous key.

Ashlee Forbes - Paramecium Drawing.jpg

Conclusions and Future Directions The hypothesis was not supported by the evidence collected in this experiment. There was a noted dearth of living organisms within the hay culture, in spite of decompositional signs. It can be concluded that the infusion would have had more protist life present had it incubated for a longer period of time. If the infusion were left for two months, for example, rather than two days, protist life would likely flourish as decomposition would be more advanced and the protists would have more of a food source and more time to reproduce.

Paramecium are well suited for this environment, having all the components of life (energy consumption and use, a distinct cell wall, storage of information) and their mode of replication is unique in that it can be both sexual and asexual. In an environment like a two-month-old hay culture, organisms like paramecium would likely be numerous, while the soil and vegetation would be greatly degraded.

To improve the experiment were I to replicate it, I would leave the hay infusion to incubate for longer. I would also take a sample from the solid layer itself since the majority of life appears to be closer to the biological material there (close to the food source) rather than on the top layer.

Notes

  • Place some notes here for visitors
  • Example: This project is currently on hold until further notice.


Recent changes

25 September 2017

     21:19 

BME100 f2017:Group12 W0800 L3‎‎ (12 changes | history) . . (+3,361). . [Priscilla Y. Han‎ (12×)]

     

21:19

(cur | prev) . . (+25). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

21:15

(cur | prev) . . (+70). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

21:11

(cur | prev) . . (-3). . Priscilla Y. Han (talk | contribs) (Experimental Design of Device)

     

21:10

(cur | prev) . . (-12). . Priscilla Y. Han (talk | contribs) (Experimental Design of Device)

     

21:10

(cur | prev) . . (+15). . Priscilla Y. Han (talk | contribs) (Experimental Design of Device)

     

21:09

(cur | prev) . . (+3,181). . Priscilla Y. Han (talk | contribs) (Experimental Design of Device)

     

21:05

(cur | prev) . . (+5). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

21:03

(cur | prev) . . (+25). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

21:01

(cur | prev) . . (+2). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

20:58

(cur | prev) . . (+29). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

20:57

(cur | prev) . . (-3). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

20:55

(cur | prev) . . (+27). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     21:19 

(Upload log). .

[Ana C. Carr‎ (4×); Priscilla Y. Han‎ (7×)]

     

21:19

. . Priscilla Y. Han (talk | contribs) uploaded File:Pulsingbargraph12.png

     

21:18

. . Priscilla Y. Han (talk | contribs) uploaded File:Mypear sonheart12.png

     

21:18

. . Priscilla Y. Han (talk | contribs) uploaded File:Heartratespree12.png

     

21:04

. . Priscilla Y. Han (talk | contribs) uploaded File:Scrnshotspree12.png

     

20:59

. . Priscilla Y. Han (talk | contribs) uploaded File:2columnbarsspree.png

     

20:56

. . Priscilla Y. Han (talk | contribs) uploaded a new version of File:Mypear sonr.png

     

20:56

. . Priscilla Y. Han (talk | contribs) uploaded File:Mypear sonr.png

     

18:36

. . Ana C. Carr (talk | contribs) uploaded a new version of File:IMG 5562.jpg(In action)

     

18:36

. . Ana C. Carr (talk | contribs) uploaded a new version of File:IMG 5562.jpg(In action)

     

18:36

. . Ana C. Carr (talk | contribs) uploaded a new version of File:IMG 5562.jpg(In action)

     

18:36

. . Ana C. Carr (talk | contribs) uploaded a new version of File:IMG 5562.jpg(In action)

     20:33 

BME100 f2017:Group8 W1030 L3‎‎ (9 changes | history) . . (+501). . [Jennifer L. Brodsky‎ (9×)]

     

20:33

(cur | prev) . . (+5). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:32

(cur | prev) . . (+112). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:29

(cur | prev) . . (0). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:28

(cur | prev) . . (+13). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:28

(cur | prev) . . (-1). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:26

(cur | prev) . . (+1). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:26

(cur | prev) . . (+177). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:25

(cur | prev) . . (+7). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:24

(cur | prev) . . (+187). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

N    20:17  BME100 f2017:Group10 W0800 L3‎ (diff | hist) . . (+767). . Whitney Hirano (talk | contribs) (Created page with "5. The mean value of heart rate calculated from the pulse ox was 98.3673 beats/min with a standard deviation of 22.2273, while the mean value of heart rate calculated from the...")
     19:56  BME100 f2017:Group9 W0800 L3‎ (diff | hist) . . (+3). . Irene Zhang (talk | contribs) (LAB 3 WRITE-UP)

     19:52 

BME100 f2017:Projects1‎‎ (3 changes | history) . . (+19). . [Yar‎; Karmella Haynes‎ (2×)]

     

19:52

(cur | prev) . . (0). . Yar (talk | contribs) (small change to force cache purge)

     

19:37

(cur | prev) . . (+21). . Karmella Haynes (talk | contribs)

     

19:11

(cur | prev) . . (-2). . Karmella Haynes (talk | contribs) (Lab 1: “Needs Identification”)

     19:19  CONJ606:Schedule‎ (diff | hist) . . (+23). . Maureen E. Hoatlin (talk | contribs) (Week-by-Week Schedule Summary)

     19:06 

BME100 f2017‎‎ (2 changes | history) . . (+1). . [Karmella Haynes‎ (2×)]

     

19:06

(cur | prev) . . (-8). . Karmella Haynes (talk | contribs)

     

19:06

(cur | prev) . . (+9). . Karmella Haynes (talk | contribs)

     17:38  User:Oww Bot‎ (diff | hist) . . (-956). . Yar (talk | contribs) (it me)

     17:36 

(User rights log). .

[Yar‎ (2×)]

     

17:36

. . Yar (talk | contribs) changed group membership for Oww Bot from bot and administrator to bot ‎

     

17:33

. . Yar (talk | contribs) changed group membership for Yeem from administrator to (none) ‎

     17:35  User:Yar‎ (diff | hist) . . (+26). . Yar (talk | contribs) (my name)

     17:34 

(Move log). .

[Yar‎ (5×)]

     

17:34

. . Yar (talk | contribs) moved page User talk:Yardena Cohen to User talk:Yar(Automatically moved page while renaming the user "Yardena Cohen" to "Yar")

     

17:34

. . Yar (talk | contribs) moved page User:Yardena Cohen/Notebook/attempt/Entry Base to User:Yar/Notebook/attempt/Entry Base(Automatically moved page while renaming the user "Yardena Cohen" to "Yar")

     

17:34

. . Yar (talk | contribs) moved page User:Yardena Cohen/Notebook/attempt to User:Yar/Notebook/attempt(Automatically moved page while renaming the user "Yardena Cohen" to "Yar")

     

17:34

. . Yar (talk | contribs) moved page User:Yardena Cohen/Notebook to User:Yar/Notebook(Automatically moved page while renaming the user "Yardena Cohen" to "Yar")

     

17:34

. . Yar (talk | contribs) moved page User:Yardena Cohen to User:Yar(Automatically moved page while renaming the user "Yardena Cohen" to "Yar")

     17:34 (User rename log) . . Yar (talk | contribs) renamed user Yardena Cohen (108 edits) to Yar(shorter)