User:Aram Kang/Notebook/Analysis of biofilm gene expression/2008/12/25

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Preparation of innoculate

  • at 3:30pm
  • Innoculate a single colony of W3110 into 10ml LB
  • Incubate it overnight at 37°C with 225 rpm
  • Preparation of LB broth--> autoclave (4:30pm)