User:Anthony Salvagno/Notebook/Research/2009/07/14/PCR to make dsDNA for stretching

From OpenWetWare
< User:Anthony Salvagno‎ | Notebook‎ | Research‎ | 2009‎ | 07‎ | 14
Jump to: navigation, search

The purpose of today's exercise is to hopefully create some tetherable dsDNA for stretching experiments. The procedure I followed is from a previous experiment of Koch's that he performed with Diego Ramallo seen here.

{{#widget:Google Spreadsheet |key=t5l0YupCCNGhrYOSkI5a44g |width=700 |height=400 }}

Thermal Cycler Program

Stage Temp (C) Time (mm:ss) Reps
1 94 4:00 1
2 94 1:00
60 1:00
72 5:00 35
3 72 9:00 1